Home Antibody All anti-CASP3 antibodies
Anti-CASP3 Antibody E83-103
Also for CASP3 (NM_032991)
|A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: Use a concentration of 5 ug/ml; WB: 1:10000; IHC-P: Use at an assay dependent dilution; IP: 1:100; FC: 1:50; ICC: 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Does not react with Rat
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - pro Caspase 3 antibody [Y83-103]; All lanes : Anti-pro Caspase 3 antibody [E83-103] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin .Predicted band size : 31 kDa.Observed band size : 35 kDa .
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma TA300422 at 1/250 dilution.
ICC/IF image of TA300422 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Anti-pro Caspase 3 antibody(TA300422); Overlay histogram showing Jurkat cells stained with TA300422 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.