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Anti-CASP3 Antibody E87
Also for CASP3 (NM_032991)
|A synthetic peptide corresponding to residues in p17 subunit of human Caspase-3 was used as immunogen. The antibody should recognize both pro-form (35kDa) and p17 cleaved-form of Caspase-3.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000; IHC-P: 1:25 - 1:50; ICC: 1:25; FC: 1:1000; IP: 1:10
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse
|Homo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta|
|CPP32; CPP32B; SCA-1|
|Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).|
MAPK signaling pathway
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Western blot - Caspase 3 antibody [E87]; All lanes : Anti-Caspase 3 [E87] antibody at 1/5000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with Camptothecin.Predicted band size : 32 kDa.Observed band size : 35 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Caspase 3 antibody [E87]; Ab32351, at a 1/25 dilution, staining Capase 3 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Flow Cytometry - Anti-Caspase 3 antibody [E87]; Overlay histogram showing Jurkat cells stained with ab32351 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.