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Home Antibody All anti-CAMK2G antibodies

Anti-CAMK2G Antibody EPR1828

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307225
  • Rabbit monoclonal antibody against CaMKII (clone EPR1828 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC420064) , 20ug
100ul $325 In Stock
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WB(1)
FC(1)
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Also for CAMK2G (NM_001222)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminal of human CaMKII was used as an immunogen.
Clone NameEPR1828 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 4
Alternative NameCAMK; CAMK-II; CAMKG
Database LinkNP_001213
FunctionCalcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. It is a ubiquitous serine/threonine protein kinase which is abundant in brain as a major PSD constituent. This enzyme is composed of distinct but related subunits (alpha, beta, gamma, and delta) (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).
Related Pathway
Melanogenesis
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - CaMKII alpha antibody [EPR1828]; All lanes : Anti-CaMKII alpha antibody [EPR1828].Lane 1 : Rat brain lysate.Lane 2 : SH SY5Y cell lysate.Lane 3 : Neuro 2a cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.
FC Image
Flow Cytometry - Anti-CaMKII alpha antibody [EPR1828]; Overlay histogram showing SH-SY5Y cells stained with TA307225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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