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Anti-CALB2 Antibody EP1798
Also for CALB2 (NM_001740)
|A synthetic peptide corresponding to residues in human Calretinin was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||FC: 1:100, ICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens calbindin 2 (CALB2), transcript variant CALB2|
|CAB29; CAL2; CR|
Entrez Gene 794 Human
Entrez Gene 12308 Mouse
Entrez Gene 117059 Rat
|Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3). |
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Western blot - Calretinin antibody [EP1798]; All lanes : Anti-Calretinin antibody [EP1798] at 1/1000 dilution.Lane 1 : Human fetal Brain lysate.Lane 2 : Mouse brain lysate.Lane 3 : Rat brain lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 29 kDa.Observed band size : 29 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calretinin antibody [EP1798]; TA307518 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calretinin antibody [EP1798]; TA307518 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Flow Cytometry - Anti-Calretinin antibody [EP1798]; Overlay histogram showing SH-SY5Y cells stained with TA307518 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.