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Home Antibody All anti-CALB2 antibodies

Anti-CALB2 Antibody EP1798

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307518 Rabbit monoclonal antibody against Calretinin (clone EPR1798 ) 100ul $325 In Stock
LC400659 CALB2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(2)
FC(1)
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Also for CALB2 (NM_001740)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Calretinin was used as an immunogen.
Clone NameEP1798 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:100, ICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1,000,
Predicted MW Explanation 29 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens calbindin 2 (CALB2), transcript variant CALB2
Alternative NameCAB29; CAL2; CR
Database LinkNP_001731
Entrez Gene 794 Human
Entrez Gene 12308 Mouse
Entrez Gene 117059 Rat
FunctionCalretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Calretinin antibody [EP1798]; All lanes : Anti-Calretinin antibody [EP1798] at 1/1000 dilution.Lane 1 : Human fetal Brain lysate.Lane 2 : Mouse brain lysate.Lane 3 : Rat brain lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 29 kDa.Observed band size : 29 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calretinin antibody [EP1798]; TA307518 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calretinin antibody [EP1798]; TA307518 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
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Flow Cytometry - Anti-Calretinin antibody; Overlay histogram showing SH-SY5Y cells stained with TA307518 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in SH-SY5Y cells under the same conditions.
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