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Home Antibody All anti-CAD antibodies

Anti-CAD Antibody EP710Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303410
  • Rabbit monoclonal antibody against CAD protein (N-term)(EP710Y)
100ul 325 Next Day
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WB(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-terminus of human CAD was used as immunogen.
Clone NameEP710Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:1000; FC: 1:100; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD)
Alternative Namemultifunctional protein CAD; CAD trifunctional protein; carbamoylphosphate synthetase 2/aspartate transcarbamylase/dihydroorotase; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Database LinkNP_004332
FunctionThe de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq].
Related Pathway

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WB Image
Western blot - CAD protein antibody [EP710Y]; Anti-CAD antibody [EP710Y] at 1/1000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg.Predicted band size : 243 kDa.Observed band size : 250 kDa .
IF Image
Immunofluorescence - CAD protein antibody [EP710Y]; Overlay histogram showing HeLa cells stained with TA303410 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
FC Image
Flow Cytometry - CAD antibody [EP710Y]; Overlay histogram showing HeLa cells stained with TA303410 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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