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Home Antibody All anti-BUB3 antibodies

Anti-BUB3 Antibody EPR5320(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310218
  • Rabbit Monoclonal antibody against Bub3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400384) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
Assay(1)
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Also for BUB3 (NM_001007793)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Bub3 was used as an immunogen.
Clone NameEPR5320(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY Suggested DilutionsWB: 1:1000 - 1:10000
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens BUB3 mitotic checkpoint protein (BUB3), transcript variant 2
Alternative NameBUB3L; hBUB3
Database LinkNP_001007794
FunctionBub3 has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. It promotes the formation of stable end-on bipolar attachments. and is necessary for kinetochore localization of BUB1. Bub3 regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20 (1).
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Bub3 antibody [EPR5320(2)]; All lanes : Anti-Bub3 antibody [EPR5320(2)] at 1/1000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 37 kDa.Observed band size : 40 kDa .
Assay Image
Other-Anti-Bub3 antibody [EPR5320(2)](TA310218); Equilibrium disassociation constant (KD)..

 

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