Anti-BUB3 Antibody, EPR5320(2)

Also for BUB3 (NM_001007793)

Gene Name	Homo sapiens BUB3 mitotic checkpoint protein (BUB3), transcript variant 2
Synonyms:	BUB3L; hBUB3
Immunogen	A synthetic peptide corresponding to residues in human Bub3 was used as an immunogen.
Buffer	Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Clone Name	EPR5320(2)	Isotype
Species Reactivity	Human, Mouse, Rat	Concentration
Purification	(Protein A or G Sepharose)
Guaranteed Application *	WB	Suggested Dilutions	WB: 1:1,000 - 10,000; IHC 1:100 - 250; ICC: 1:100 - 250; IP: 1:10 - 100; FC: 1:10 - 100;
Background	Bub3 has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. It promotes the formation of stable end-on bipolar attachments. and is necessary for kinetochore localization of BUB1. Bub3 regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20 (1).
Related Pathway	Cell cycle

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WB Image

Western blot analysis on (A) HeLa, (B) Jurkat, and (C) 293T cell lysates using anti-Bub3 RabMAb.

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Anti-BUB3 Antibody EPR5320(2)

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