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Home Antibody All anti-BUB1B antibodies

Anti-BUB1B TRUEMAB Antibody clone 6E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500679
  • BuB1B (BubR1) mouse monoclonal antibody, clone 6E3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420068) , 20ug Explanation
100ul 379 In Stock
CF500679
  • Carrier-free (BSA/glycerol-free) BuB1B mouse monoclonal antibody, clone 6E3
100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(3)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.
Clone Nameclone 6E3 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.4 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Alternative NameBub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Database LinkNP_001202
Entrez Gene 701 Human
Entrez Gene 171576 Rat
Entrez Gene 100423079 Monkey
Entrez Gene 608350 Dog
FunctionThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq].
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
IF Image
Anti-BUB1B mouse monoclonal antibody (TA500679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).
FC Image
HEK293T cells transfected with either RC205844 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BUB1B antibody(TA500679), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody(TA500679),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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