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Anti-BUB1B TRUEMAB Antibody clone 11G2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BUB1B (NM_001211)
|Full-length protein expressed in 293T cell transfected with human BUB1B expression vector|
|WB, IHC, IF
||WB 1:2000 IHC 1:50 IF 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)|
|Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1|
|This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500540, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500540, Dilution 1:50)
Anti-BUB1B mouse monoclonal antibody (TA500540) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).