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Anti-BTK TRUEMAB Antibody Clone OTI10E10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501118 anti-BTK mouse monoclonal antibody, clone OTI10E10 (formerly 10E10) 100ul
In Stock
LC424947 BTK HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501118 Carrier-free (BSA/glycerol-free) BTK mouse monoclonal antibody, clone OTI10E10 (formerly 10E10) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.
Clone NameClone OTI10E10 IsotypeIgG2a
Species ReactivityHuman Concentration0.87 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 76.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Bruton agammaglobulinemia tyrosine kinase (BTK), transcript variant 1
Alternative NameAGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA
Database LinkNP_000052
Entrez Gene 695 Human
FunctionThe protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement.
Related PathwayProtein KinaseDruggable Genome B cell receptor signaling pathwayFc epsilon RI signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BTK monoclonal antibody at 1:200 dilution.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BTK monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501118, Dilution 1:50)
Anti-BTK mouse monoclonal antibody (TA501118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK(RC211582).
Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody (TA501118).
Flow cytometry analysis of monocytes from non-XLA or XLA patients using anti-BTK antibody (TA501118). Image courtesy: Dr. H. Kanaegane, University of Toyama, Japan.
Figure from citation: Flow Cytometry analysis of intracellular BTK expression by using anti-BTK antibody (clone OTI10E10) in CD14+ monocytes. Black histograms indicate BTK expression and Gray histograms indicate isotype control. BTK expression in the patient was reduced compared with that in a normal control. View Citation


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