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Anti-BTK TRUEMAB Antibody clone 12H4
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:500, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
Immunohistochemical staining of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501091, Dilution 1:50)
Anti-BTK mouse monoclonal antibody (TA501091) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK(RC211582).
HEK293T cells transfected with either pCMV6-ENTRY BTK(RC211582)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BTK mouse monoclonal(TA501091), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-BTK antibody(TA501091),(Red), compared to a nonspecific negative control antibody,(Blue).