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Home Antibody All anti-BTK antibodies

Anti-BTK Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323749
  • Anti-BTK Rabbit Polyclonal Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424947) , 20ug Explanation
100ul 325 2-3 Weeks
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WB(1)
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Also for BTK (NM_000061)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFusion protein corresponding to a region derived from 30-240 amino acids of human Bruton agammaglobulinemia tyrosine kinase
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration2.43mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-2000
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens Bruton agammaglobulinemia tyrosine kinase (BTK), transcript variant 1
Alternative NameAGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA
Database LinkNP_000052
Entrez Gene 695 Human
Entrez Gene 12229 Mouse
Entrez Gene 367901 Rat
FunctionBruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCß through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation. The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity.
Related PathwayProtein KinaseDruggable Genome B cell receptor signaling pathwayFc epsilon RI signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 76 kDa . Positive control: Daudi cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

 

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