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Home Antibody All anti-BRD4 antibodies

Anti-BRD4 Antibody EPR5150(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310215
  • Rabbit Monoclonal antibody against BRD4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC415375) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human BRD4 was used as an immunogen.
Clone NameEPR5150(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500
Predicted MW Explanation 152 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens bromodomain containing 4 (BRD4), transcript variant short
Alternative NameCAP; HUNK1; HUNKI; MCAP
Database LinkNP_055114
Entrez Gene 23476 Human
Entrez Gene 57261 Mouse
Entrez Gene 362844 Rat
FunctionBRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif that may be involved in chromatin targeting. BRD4 has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people (1).
Related PathwayProtein Kinase

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Brd4 antibody [EPR5150(2)]; All lanes : Anti-Brd4 antibody [EPR5150(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Caco2 cell lysate.Lane 3 : T.T. cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 152 kDa.
Assay Image
Other-Anti-Brd4 antibody [EPR5150(2)](TA310215); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)]; TA310215 at 1/100 dilution staining Brd4 in Human colon carcinoma tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)]; TA310215 (1/500) staining Brd4 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counter stained with DAPI in order to highlight the nucleus (red).

 

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