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Anti-BRD4 Antibody EPR5150(2)
Also for BRD4 (NM_014299)
|A synthetic peptide corresponding to residues in human BRD4 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens bromodomain containing 4 (BRD4), transcript variant short|
|CAP; HUNK1; HUNKI; MCAP|
Entrez Gene 23476 Human
Entrez Gene 57261 Mouse
Entrez Gene 362844 Rat
|BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif that may be involved in chromatin targeting. BRD4 has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people (1). |
|Protein Kinase |
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Western blot - Anti-Brd4 antibody [EPR5150(2)]; All lanes : Anti-Brd4 antibody [EPR5150(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Caco2 cell lysate.Lane 3 : T.T. cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 152 kDa.
Other-Anti-Brd4 antibody [EPR5150(2)](TA310215); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)]; TA310215 at 1/100 dilution staining Brd4 in Human colon carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [EPR5150(2)]; TA310215 (1/500) staining Brd4 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counter stained with DAPI in order to highlight the nucleus (red).