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Home Antibody All anti-BRAF antibodies

Anti-BRAF TRUEMAB Antibody Clone OTI1D4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500852 anti-BRAF mouse monoclonal antibody, clone OTI1D4 (formerly 1D4) 100ul 325
2 Weeks
LC401382 BRAF HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500852 Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody, clone OTI1D4 (formerly 1D4) 100ug $450 3 Days
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OriGene Data

ImmunogenFull length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
Clone NameClone OTI1D4 IsotypeIgG1
Species ReactivityHuman Concentration0.64 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:2000, IF 1:100, Flow 1:100
Predicted MW Explanation 84.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
Entrez Gene 673 Human
FunctionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
Anti-BRAF mouse monoclonal antibody (TA500852) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).
Immunoprecipitation(IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either pCMV6-ENTRY BRAF(RC211013)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BRAF mouse monoclonal(TA500852), and then analyzed by flow cytometry.


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