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Home Antibody All anti-BRAF antibodies

Anti-BRAF TRUEMAB Antibody clone 3A7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500440
  • anti-BRAF mouse monoclonal antibody,clone 3A7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401382) , 20ug
100ul 325 In Stock
CF500440
  • Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody,clone 3A7
100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human BRAF expression vector
Clone Nameclone 3A7 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration1.28mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000; IHC 1:50; IF 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
FunctionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this ge
Related Pathway
Focal Adhesion
MAPK signaling pathway
Senescence and Autophagy

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500440, Dilution 1:50)
IF Image
Anti-BRAF mouse monoclonal antibody (TA500440) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).

 

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