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Home Antibody All anti-BRAF antibodies

Anti-BRAF TRUEMAB Antibody clone 3A7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500440
  • anti-BRAF mouse monoclonal antibody,clone 3A7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401382) , 20ug Explanation
100ul 325 In Stock
CF500440
  • Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody,clone 3A7
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IP(1)
IHC(5)
IF(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human BRAF expression vector
Clone Nameclone 3A7 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration1.2 mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:2000; IHC 1:50; IF 1:100
Predicted MW Explanation 84.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
Entrez Gene 673 Human
Entrez Gene 114486 Rat
Entrez Gene 475526 Dog
FunctionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this ge
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded colon tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500440, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500440, Dilution 1:50)
IF Image
Anti-BRAF mouse monoclonal antibody (TA500440) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).
IP Image
Immunoprecipitation(IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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