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Anti-BRAF PHOSPHO Antibody
Also for BRAF (NM_004333)
|The antiserum was produced against synthesized phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-SP-S-D).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)|
|B-RAF1; BRAF1; NS7; RAFB1|
MAPK signaling pathway
Senescence and Autophagy
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Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from K562 cells (Lane 2), JK cells (Lane 3) and 293 cells (Lane 4), using B-RAF (Phospho-Ser446) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody.The picture on the right is treated with the synthesized peptide.