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Anti-BRAF PHOSPHO Antibody
Also for BRAF (NM_004333)
|The antiserum was produced against synthesized phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-SP-S-D).|
||WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Homo sapiens v-raf murine sarcoma viral oncogene homolog B (BRAF)|
|B-RAF1; BRAF1; NS7; RAFB1|
MAPK signaling pathway
Senescence and Autophagy
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Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from K562 cells (Lane 2), JK cells (Lane 3) and 293 cells (Lane 4), using B-RAF (Phospho-Ser446) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody.The picture on the right is treated with the synthesized peptide.