Home Antibody All anti-BRAF antibodies
Also for BRAF (NM_004333)
|This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from the Central region of human B-RAF.|
|Human (Predicted: Mouse, Chicken)
|WB, IHC, IF, FC
||IF: 1:10~50, WB: 1:1000, IHC: 1:10~50, FC: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Western blot analysis of BRAF Antibody (Center) polyclonal antibody(Cat.#TA302165)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center)(Cat.#TA302165), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of C2C12 cell stained with BRAF Antibody (Center)(Cat#TA302165).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37Ã¢). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37Ã¢).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37Ã¢).BRAF immunoreactivity is localized to Cytoplasm significantly.
B-RAF Antibody (Center) (Cat. #TA302165) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.