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Home Antibody All anti-BRAF antibodies

Anti-BRAF Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302165
  • Rabbit Polyclonal Antibody against BRAF (Center)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401382) , 20ug
100ug 325 In Stock
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WB(1)
IHC(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human BRAF.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
FunctionBRAF, a mebmer of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Related Pathway
Focal Adhesion
MAPK signaling pathway
Senescence and Autophagy

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of BRAF Antibody (Center) polyclonal antibody(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

 

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