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Home Antibody All anti-BRAF antibodies

Anti-BRAF PHOSPHO Antibody EPR2208Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301156
  • Rabbit Monoclonal Antibody against BRAF (Clone EPR2208Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 401 of human B-Raf was used as an immunogen. This antibody detects B-Raf phosphorylated on threonine 401.
Clone NameEPR2208Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1: 1000~5000; ICC: 1: 100; IP: 1:20
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
FunctionB-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.
Related Pathway
Focal Adhesion
MAPK signaling pathway
Senescence and Autophagy

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WB Image
Western blot - B Raf antibody [EPR2208Y]; All lanes : Anti-B Raf (phospho T401) antibody [EPR2208Y] at 1/5000 dilution.Lane 1 : PC-12 cell lysates (cells were untreated).Lane 2 : PC-12 cell lysates (cells were treated with TPA) .Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.

 

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