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Anti-BRAF Antibody EP152Y
Also for BRAF (NM_004333)
|A synthetic peptide corresponding to residues in human B-Raf was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; FC: 1:1000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC.
|Homo sapiens v-raf murine sarcoma viral oncogene homolog B (BRAF)|
|B-RAF1; BRAF1; NS7; RAFB1|
|B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma. |
MAPK signaling pathway
Senescence and Autophagy
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Western blot - B Raf antibody [EP152Y]; Anti-B Raf antibody [EP152Y] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 85 kDa.Observed band size : 87 kDa .
Western blot - B Raf antibody [EP152Y]; All lanes : Anti-B Raf antibody [EP152Y] at 1/1000 dilution.Lane 1 : Lysate prepared from mouse brain.Lane 2 : Lysate prepared from mouse heart.Lane 3 : Lysate prepared from mouse kidney.Lane 4 : Lysate prepared from mouse spleen.Lane 5 : Lysate prepared from rat brain.Lane 6 : Lysate prepared from rat heart.Lane 7 : Lysate prepared from rat kidney.Lane 8 : Lysate prepared from rat spleen.Predicted band size : 85 kDa.Exposure time : 3 minutes
Immunohistochemistry (Paraffin-embedded sections) - B Raf antibody [EP152Y]; This image shows paraffin embedded human prostate cancer tissue sample stained with ab33899 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - B Raf antibody [EP152Y]; ab33899 staining B Raf cells from human prostate tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed and permeabilized in PBS-Tween 20 prior to blocking in 70% serum for 10 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 1 hour at 25°C. A biotin conjugated goat polyclonal to mouse Ig was used as the secondary.
Flow Cytometry - Anti-B Raf antibody [EP152Y]; Overlay histogram showing SH-SY5Y cells stained with ab33899 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.