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BMP4 UltraMAB Antibody, Clone UMAB42
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for BMP4 (NM_130850)
|Homo sapiens bone morphogenetic protein 4 (BMP4), transcript variant 2|
|BMP2B; BMP2B1; MCOPS6; OFC11; ZYME|
|Recombinant fragment expressed in E.coli corresponding to amino acids 293-408 of human BMP4|
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
||0.5~1.0 mg/ml (Lot Dependent)
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|WB, IHC, 10K-CHIP
||WB 1:500, IHC 1:100
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HEK293T cells transfected pCMV6-ENTRY BTRC (RC219979, Left lane) cDNA for 48 hrs or HEK293T cells untransfected (Right lane) were lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-BMP4 monoclonal antibody (clone UMAB42, 1:500)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human breast using anti-BMP4 mouse monoclonal antibody. (Clone UMAB38 , dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human normal pancreas and Carcinoma of Human pancreas using anti-BMP4 mouse monoclonal antibody. (Clone UMAB38 , dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human melanoma using anti-BMP4 mouse monoclonal antibody; an unrelated antibody of the same isotype was used as negative control. (Clone UMAB38 , dilution 1:50; heat-induced epitope retrieval by 10mM citri
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BMP4 mouse monoclonal antibody (Clone UMAB38). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-BMP4 (Clone UMAB38) very specifically recognizes BMP4 antigen on OriGene protein microarray chip.