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Home Antibody All anti-BIRC3 antibodies

Anti-BIRC3 Antibody E40

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300345
  • Rabbit Monoclonal Antibody against BIRC3 (Clone E40)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420093) , 20ug
100ul 325 3-7 Days
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WB(1)
IP(1)
IHC(1)
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Also for BIRC3 (NM_001165)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues, before the breakpoint for translocation to form BIRC3-MALT1, of Human BIP3 was used as immunogen. The antibody does not cross-react with other IAP family members.
Clone NameE40 IsotypeIgG fraction
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:100; IP: Use a concentration of 5 ug/ml
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens baculoviral IAP repeat containing 3 (BIRC3), transcript variant 1
Alternative NameAIP1; API2; c-IAP2; CIAP2; HAIP1; HIAP1; MALT2; MIHC; RNF49
Database LinkNP_001156
FunctioncIAP-2, also known as HIAP-1, is a member of the inhibitor of apoptosis (IAP) family of proteins. These proteins contain a BIR (baculovirus IAP repeat) domain near the amino-terminus (1). cIAP-2 is localized to the cytoplasm. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form a heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). This complex is thought to play a role in inhibition of apoptosis (2,3). cIAP-2 inhibits cytochrome c-induced activation of caspase-9, thereby preventing initiation of the caspase cascade and apoptosis (4).
Related Pathway
Apoptosis
Focal Adhesion

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - cIAP2 antibody [E40]; Anti-cIAP2 antibody [E40] at 1/1000 dilution + Daudi cell lysate.Predicted band size : 72 kDa.Observed band size : 72 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - cIAP2 antibody [E40]; TA300345 at a 1:50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
IP Image
Immunoprecipitation - Anti-cIAP2 antibody [E40]; cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5ug of Rabbit polyclonal to cIAP2 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300345.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 72kDa; cIAP2

 

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