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UltraMAB

BID UltraMAB Antibody, Clone UMAB139

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (1) Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500101
  • BID mouse monoclonal antibody,clone UMAB139, 100 ul
$468 In Stock Add to Shopping Cart
UM570101
  • BID mouse monoclonal antibody,clone UMAB139, 30 ul
$120 In Stock Add to Shopping Cart
CE IVD Logo CE marked antibody of this clone also available.
WB(4)
Data Img
IHC(5)
Data Img
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OriGene Data

ImmunogenFull length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
Clone NameClone UMAB139 IsotypeIgG2b
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens BH3 interacting domain death agonist (BID), transcript variant 2
Alternative NameFP497
Database LinkNP_001187
FunctionThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of  HEK293 cell lysate (35ug) by using anti-BID monoclonal antibody.
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Western blot analysis of  COLO205 cell lysate (35ug) by using anti-BID monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BID monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of DU145 cell lysate (35ug) by using anti-BID monoclonal antibody UM500101
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human kidney carcinoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
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Immunohistochemical staining of paraffin-embedded human melanoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
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Immunohistochemical staining of paraffin-embedded of 3 human colon cancers using anti-BID clone UMAB139 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500101 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The composit image of 3 colon cancers labeled A, B, and C shows strong membranous and cytoplasmic staining in >75 % of tumor cells and image A and B shows <10% nuclear stain.
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