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Anti-BID TRUEMAB Antibody Clone 3C8
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.|
|WB, IHC, IF
|| WB 1:400~4000, IHC 1:150, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2|
|This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506768)
Anti-BID mouse monoclonal antibody (TA506768) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID(RC207261).