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Home Antibody All anti-BHMT antibodies

Anti-BHMT TRUEMAB Antibody Clone 1E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500961
  • anti-BHMT mouse monoclonal antibody, clone 1E5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400644) , 20ug Explanation
100ul $325 In Stock
CF500961
  • Carrier-free (BSA/glycerol-free) BHMT mouse monoclonal antibody, clone 1E5
100ug $450 3-4 weeks
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WB(1)
IHC(3)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
Clone NameClone 1E5 IsotypeIgG2a
Species ReactivityHuman Concentration0.7 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:50, Flow 1:100
Predicted MW Explanation 45kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens betaine--homocysteine S-methyltransferase (BHMT)
Alternative NameBHMT1; HEL-S-61p
Database LinkNP_001704
Entrez Gene 635 Human
FunctionThis gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Related Pathway Glycine, serine and threonine metabolismCysteine and methionine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
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Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500961, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500961, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500961, Dilution 1:50)
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HEK293T cells transfected with either pCMV6-ENTRY BHMT(RC203148)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BHMT mouse monoclonal(TA500961), and then analyzed by flow cytometry.
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