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Home Antibody All anti-BHMT antibodies

Anti-BHMT TRUEMAB Antibody Clone OTI3E11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500959 anti-BHMT mouse monoclonal antibody, clone OTI3E11 (formerly 3E11) 100ul $379 In Stock
LC400644 BHMT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500959 Carrier-free (BSA/glycerol-free) BHMT mouse monoclonal antibody, clone OTI3E11 (formerly 3E11) 100ug $450 3-4 weeks
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WB(2)
IHC(4)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
Clone NameClone OTI3E11 IsotypeIgG2a
Species ReactivityHuman Concentration0.93 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions IHC 1:200, WB: 1:200
Predicted MW Explanation 45kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens betaine--homocysteine S-methyltransferase (BHMT)
Alternative NameBHMT1; HEL-S-61p
Database LinkNP_001704
Entrez Gene 635 Human
FunctionThis gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Related Pathway Glycine, serine and threonine metabolismCysteine and methionine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-BHMT monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500959, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500959, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500959, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500959, Dilution 1:50)
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Anti-BHMT mouse monoclonal antibody (TA500959) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT(RC203148).
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HEK293T cells transfected with either pCMV6-ENTRY BHMT(RC203148)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BHMT mouse monoclonal(TA500959), and then analyzed by flow cytometry.
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