Homo sapiens betaine--homocysteine S-methyltransferase (BHMT)
Synonyms:
BHMT1
Immunogen
Peptide with sequence C-EQQLKELFEKQK, from the C Terminus of the protein sequence according to NP_001704.1.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clone Name
Isotype
Goat IgG
Species Reactivity
Human, Mouse, Rat, Dog
Concentration
0.5 mg/ml
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
ELISA: 1:16,000. WB: 0.03-0.1µg/ml.
Background
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq].
Related Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY BHMT (RC203148) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
