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Anti-BEST3 TRUEMAB Antibody clone 3D7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BEST3 (NM_152439)
|Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens bestrophin 3 (BEST3), transcript variant 2|
|BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BEST3 (RC218436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BEST3.
Anti-BEST3 mouse monoclonal antibody (TA502154) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436).
HEK293T cells transfected with either RC218436 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BEST3 antibody(TA502154), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-BEST3 antibody(TA502154),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).