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Home Antibody All anti-BECN1 antibodies

Anti-BECN1 TRUEMAB Antibody Clone OTI3H8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA502517 BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI3H8 (formerly 3H8) 100ul $325 In Stock
LC401239 BECN1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502517 Carrier-free (BSA/glycerol-free) BECN1 mouse monoclonal antibody, clone OTI3H8 (formerly 3H8) 100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(1)
FC(1)
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Also for BECN1 (NM_003766)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.
Clone NameClone OTI3H8 IsotypeIgG2a
Species ReactivityHuman , Rat , Monkey Concentration0.55 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 51.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens beclin 1, autophagy related (BECN1)
Alternative NameATG6; beclin1; VPS30
Database LinkNP_003757
Entrez Gene 8678 Human
Entrez Gene 114558 Rat
Entrez Gene 711649 Monkey
FunctionBeclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010].
Related PathwayDruggable Genome Regulation of autophagy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502517)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502517)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502517)
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Anti-BECN1 mouse monoclonal antibody (TA502517) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1(RC201629).
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HEK293T cells transfected with either RC201629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BECN1 antibody(TA502517), and then analyzed by flow cytometry.
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