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Anti-BDNF Antibody EP1293


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SKU Description Amount Price Availability*  
TA307522 Rabbit monoclonal antibody against BDNF(clone EP1293) 100ul $325 2 Weeks
LC400640 BDNF HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human BDNF was used as an immunogen.
Clone NameEP1293 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
Predicted MW Explanation 28 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 4
Alternative NameANON2; BULN2
Database LinkNP_001700
Entrez Gene 627 Human
Entrez Gene 12064 Mouse
Entrez Gene 24225 Rat
FunctionBrain-derived neurotrophic factor (BDNF), like other neurotrophins, is a polypeptide factor initially regarded to be responsible for neuron proliferation, differentiation and survival through its uptake at nerve terminals and retrograde transport to the cell body (1). BDNF has been linked to a variety of neurological and psychological conditions such as Huntington’s disease, Alzheimer’s disease, depression and anxiety. Studies show that transgenic overexpression of the neurotrophin BDNF has an unexpected facilitatory effect on anxiety-like behavior, concomitant with increased spinogenesis in the basolateral amygdala (2). In addition, BDNF enhances the neurite outgrowth on immature astrocytes. A multifactorial strategy based on transplantation of neonatal astrocytes in the presence of additional BDNF may stimulate axon regrowth after experimental injury to the central nervous system (3). The precursor pro-BDNF is 27-34 kDa before proteolytic cleavage, whereas the mature form of BDNF is 14 kDa.
Related PathwaySecreted ProteinES Cell Differentiation/IPSTransmembraneAdult stem cellsEmbryonic stem cellsInduced pluripotent stem cellsDruggable Genome MAPK signaling pathwayNeurotrophin signaling pathwayHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - BDNF antibody [EP1293]; All lanes : Anti-BDNF antibody [EP1293] at 1/1000 dilution.Lane 1 : C6 cell lysate.Lane 2 : Fetal brain lysate.Lane 3 : Human hippocampus lysate.Lane 4 : Recombinant BDNF protein.Lysates/proteins at 10 ug per lane.Predicted band size : 28 kDa.
Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EP1293]; TA307522 staining BDNF in human lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.1% Saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with TA307522 at a 1/100 dilution for 14 hours at 4°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - BDNF antibody [EP1293]; Immunofluorescent staining of PC12 cells using TA307522 at a dilution of 1/100.


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