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Anti-BCL2A1 Antibody EP517Y
Also for BCL2A1 (NM_004049)
|A synthetic peptide corresponding to residues in the N-term of human Bcl-2 related protein A1 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:500; IHC-P: Use at an assay dependent dilution; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens BCL2-related protein A1 (BCL2A1), transcript variant 1|
|ACC-1; ACC-2; BCL2L5; BFL1; GRS; HBPA1|
|This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - A1 antibody [EP517Y]; Anti-Bcl2A1 antibody [EP517Y] at 1/500 dilution + Raji cell lysate.Predicted band size : 20 kDa.Observed band size : 27 kDa .
Immunohistochemistry (Paraffin-embedded sections) - A1 antibody [EP517Y]; ab33862, at a 1/50 dilution, staining human adenocarcinoma by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Bcl2A1 antibody [EP517Y]; ab33862 at 1/100, staining Hela cell lysate by Immunofluorescent.
Flow Cytometry - Bcl2A1 antibody [EP517Y]; Overlay histogram showing HepG2 cells stained with ab33862 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.