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Home Antibody All anti-BCL2 antibodies

Anti-BCL2 Antibody E17

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303400
  • Rabbit monoclonal antibody against Bcl-2(E17)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424602) , 20ug
100ul 325 In Stock
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WB(1)
IHC(4)
FC(1)
Assay(1)
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Also for BCL2 (NM_000633)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen. The antibody does not cross-react with other Bcl-2 family members.
Clone NameE17 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:200; FC: 1:200; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Does not react with Rat

Reference Data

Target NameHomo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha
Alternative NameBcl-2; PPP1R50
Database LinkNP_000624
FunctionThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq].
Related Pathway
Apoptosis
Focal Adhesion

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Bcl2 antibody [E17]; Anti-Bcl-2 antibody [E17] at 1/1000 dilution + Jurkat cell lysate .Predicted band size : 26 kDa.Observed band size : 26 kDa.
Assay Image
Other-Anti-Bcl2 antibody [E17](TA303400); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody [E17]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA303400 at 1/200 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2 antibody [E17]; TA303400 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2 antibody [E17]; TA303400 showing positive staining in B cell lymphoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2 antibody [E17]; Immunohistochemical analysis of Human salivary glands taken from patients with primary Sj?gren's syndrome, staining Bcl2 with TA303400. Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked using 2% BSA, 10% normal serum and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100) for one hour at room temperature. An AlexaFluor?594-conjugated anti-rabbit IgG was used as the secondary antibody. N.B. Panels B and D are higher magnifications of panels A and C, respectively.
FC Image
Flow Cytometry-Anti-Bcl2 antibody [E17](TA303400); Overlay histogram showing Jurkat cells stained with TA303400 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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