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Home Antibody All anti-BCKDK antibodies

Anti-BCKDK TRUEMAB Antibody Clone OTI11C9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500685 BCKDK (BCKDH kinase) mouse monoclonal antibody, clone OTI11C9 (formerly 11C9) 100ul $379 In Stock
LC401780 BCKDK HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500685 Carrier-free (BSA/glycerol-free) BCKDK mouse monoclonal antibody, clone OTI11C9 (formerly 11C9) 100ug $450 3-4 weeks
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WB(1)
IHC(2)
IF(1)
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Also for BCKDK (NM_005881)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.
Clone NameClone OTI11C9 IsotypeIgG1
Species ReactivityHuman Concentration0.8 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 46.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 1
Alternative NameBCKDKD; BDK
Database LinkNP_005872
Entrez Gene 10295 Human
FunctionCatalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex .
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (RC203601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-BCKDK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500685)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BCKDK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500685)
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Anti-BCKDK mouse monoclonal antibody (TA500685) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK(RC203601).
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