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BBOX1 UltraMAB Antibody, Clone Clone UMAB120
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for BBOX1 (NM_003986)
|Full length human recombinant protein of human BBOX1 (NP_003977) produced in HEK293T cell.|
|Clone Clone UMAB120
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
|| IHC 1:100~200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1)|
|BBH; BBOX; G-BBH; gamma-BBH|
|This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]. |
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Uterus, 3: Omentum, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:250
Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500085 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The composit image shows strong cytoplasmic, membrane, and nuclear in panel A tumor cells and no staining in panel B tumor cells but strong staining is normal tubule epithelial cells..
Immunohistochemical staining of paraffin-embedded human liver using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500085 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic and membrane in the hepatocytes
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500085; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500085; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (UM500085, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).