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Home Antibody All anti-BAX antibodies

Anti-BAX Antibody E63

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303399
  • Rabbit monoclonal antibody against Bax(E63)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC408505) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
ELISA(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the vicinity of the dimerization domain of human Bax was used as immunogen. The antibody should recognize cytoplasmic isoform beta, delta, and membrane isoform alpha of Bax. The antibody does not cross-reac
Clone NameE63 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ELISA, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent concentration; IP: 1:100; FC: 1:1000; Sandwich ELISA: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens BCL2-associated X protein (BAX), transcript variant delta
Alternative NameBCL2L4
Database LinkNP_620118
FunctionThe protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq].
Related Pathway
Apoptosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Bax antibody [E63]; Anti-Bax antibody [E63] at 1/2000 dilution + HeLa cell lysate.Secondary.Goat anti-rabbit HRP antibody.developed using the ECL technique.Predicted band size : 21 kDa.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
ELISA Image
Sandwich ELISA - Bax antibody [E63]; Standard Curve for Bax (Analyte: dilution range 1pg/ml to 1ug/ml using Capture Antibody at 0.2ug/ml and Detector Antibody at 0.5ug/ml Concentration of may vary from lot to lot; please use this curve as guideline.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63]; Immunohistochemical analysis of paraffin-embedded Human lymph node using anti-Bax Rabbit Monoclonal Antibody at 1/250 dilution.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
FC Image
Flow Cytometry - Anti-Bax antibody [E63]; Overlay histogram showing HeLa cells stained with TA303399 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>

 

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