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Anti-BAG1 Antibody Y166
Also for BAG1 (NM_004323)
|A synthetic peptide corresponding to residues following the repeat 7 of human Bag-1 was used as immunogen. The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens BCL2-associated athanogene (BAG1), transcript variant 1|
|BAG-1; HAP; RAP46|
|The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites. [provided by RefSeq]. |
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Western blot - Bag1 antibody [Y166]; Anti-Bag1 antibody [Y166] at 1/500 dilution + HeLa cell lysate.Predicted band size : 31 kDa.Observed band size : 33 kDa .Additional bands at : 46 kDa (possible isoform),50 kDa (possible isoform).
Immunohistochemistry (Paraffin-embedded sections) - Bag1 antibody [Y166]; Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Bag1 antibody [Y166]; Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.