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Home Antibody Antitag Antibodies

Anti-tag Antibodies

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OriGene has developed a family of anti-tag antibodies to optimize the detection and purification of the recombinant proteins. Most of OriGene's anti-tag antibodies are mouse monoclonal antibodies which have been screened and extensively validated for high sensitivity and specificity.

Save $100 when anti-tGFP (TA150041) ordered with a GFP-tagged ORF clone (RG2xxxxx)

Fusion Tag
DDK GST HA His Myc V5
Fluorescent Protein Tag
eGFP tGFP mCherry tdTomato More FP Tags
Tag Description Applications SKU Size Price  
ZsGreen1 Mouse monoclonal ZsGreen1 antibody, Magnetic beads IP, Protein Purification TA183023 1ml $318 Add to Shopping Cart
ZsYello1 Mouse monoclonal ZsYellow1 antibody, Magnetic beads IP, Protein Purification TA183024 1ml $318 Add to Shopping Cart
DsRed2 Mouse monoclonal DsRed2 antibody, Magnetic beads IP, Protein Purification TA183025 1ml $318 Add to Shopping Cart
Timer Mouse monoclonal Timer antibody, Magnetic beads IP, Protein Purification TA183026 1ml $318 Add to Shopping Cart
tdTomato Mouse monoclonal tdTomato antibody, Magnetic beads IP, Protein Purification TA183027 1ml $318 Add to Shopping Cart
AcGFP1 Mouse monoclonal AcGFP1/PS-CFP2 antibody, Magnetic beads IP, Protein Purification TA183028 1ml $318 Add to Shopping Cart
DsRed2-Express2 Mouse monoclonal DsRed-Express2 antibody, Magnetic beads IP, Protein Purification TA183029 1ml $318 Add to Shopping Cart
mCherry Mouse monoclonal mcherry antibody, Magnetic beads IP, Protein Purification TA183030 1ml $318 Add to Shopping Cart
mRaspberry Mouse monoclonal mRaspberry/AmCyan1 antibody, Magnetic beads IP, Protein Purification TA183031 1ml $318 Add to Shopping Cart
mBanana Mouse monoclonal mBanana antibody, Magnetic beads IP, Protein Purification TA183032 1ml $318 Add to Shopping Cart
mStrawberry Mouse monoclonal mStrawberry antibody, Magnetic beads IP, Protein Purification TA183033 1ml $318 Add to Shopping Cart
eRFP Mouse monoclonal eRFP antibody, Magnetic beads IP, Protein Purification TA183034 1ml $318 Add to Shopping Cart
mOrange Mouse monoclonal mOrange/mOrange2 antibody, Magnetic beads IP, Protein Purification TA183035 1ml $318 Add to Shopping Cart
eCFP Mouse monoclonal eCFP antibody, Magnetic beads IP, Protein Purification TA183036 1ml $318 Add to Shopping Cart
mPlum Mouse monoclonal mPlum antibody, Magnetic beads IP, Protein Purification TA183037 1ml $318 Add to Shopping Cart
mGFP Mouse monoclonal mGFP/mCFP/mYFP/KillerRed antibody, Magnetic beads IP, Protein Purification TA183038 1ml $318 Add to Shopping Cart
DsRed-Monomer Mouse monoclonal DsRed-Monomer antibody, Magnetic beads IP, Protein Purification TA183039 1ml $318 Add to Shopping Cart
E2-Crimson Mouse monoclonal E2-Crimson antibody, Magnetic beads IP, Protein Purification TA183040 1ml $318 Add to Shopping Cart
mKate Mouse monoclonal mKate antibody, Magnetic beads IP, Protein Purification TA183041 1ml $318 Add to Shopping Cart
mBFP Mouse monoclonal mBFP antibody, Magnetic beads IP, Protein Purification TA183042 1ml $318 Add to Shopping Cart
mRFP Mouse monoclonal mRFP antibody, Magnetic beads IP, Protein Purification TA183043 1ml $318 Add to Shopping Cart
Dendra2 Mouse monoclonal Dendra2 antibody, Magnetic beads IP, Protein Purification TA183044 1ml $318 Add to Shopping Cart
AsRed2 Mouse monoclonal AsRed2 antibody, Magnetic beads IP, Protein Purification TA183045 1ml $318 Add to Shopping Cart
tGFP Anti-tGFP magnetic beads, 1ml IP, Protein purification TA150039 1ml $318 Add to Shopping Cart
tGFP Anti-tGFP magnetic beads, 5ml IP, Protein purification TA150040 5ml $968 Add to Shopping Cart
Myc 9E10, Anti-Myc Magnetic beads IP, Protein Purification TA150044 1ml $318 Add to Shopping Cart
Myc 9E10, Anti-Myc Magnetic beads IP, Protein Purification TA150045 5ml $968 Add to Shopping Cart
DDK 4C5, Anti-DDK Magnetic beads IP, Protein Purification TA150042 1ml $318 Add to Shopping Cart
DDK 4C5, Anti-DDK Magnetic beads IP, Protein Purification TA150036 5ml $968 Add to Shopping Cart
* For bulk antibody package, please contact Tech Support
** Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C; Flag® is a trademark of Sigma-Aldrich.

Features of OriGene Antitag Antibodies

  • 4C5 Anti-DDK Antibody

    OriGene’s anti-tag antibodies are developed with high standards of production procedure and validation. For example, 4C5 anti-DDK antibody is generated to detect the DYKDDDDK epitope (same epitope as Flag®). This antibody has been verified for Western blot, immunoprecipitation, immunofluorescence and flow cytometry. Other features include:

    • Higher sensitivity and specificity compared to other vendors’ anti-flag antibodies
    • Can be used to purify recombinant proteins with high purities and yields
    • Can also be used to detect recombinant proteins with low expression levels

    4C5 anti-DDK antibody has already been applied to

    • Validate the recombinant protein expression from more than 15,000 OriGene TrueORF cDNA clones and VERIFY overexpression lysates
    • Purify more than 5,000 human recombinant proteins from HEK293 cells
    • Purify immunogens under native conditions for the development of TrueMAB antibodies
    AntiDDK DDK Comparison Image

    4C5 anti-DDK monoclonal antibody (TA50011) showed higher sensitivity in the side-by-side comparison with M2 monoclonal antibody from vendor S. Both antibodies were used at 1:2000 dilution against same sample (TP53) dilutions.

  • 2H8 Anti-tGFP Antibody

    2H8 anti-tGFP is another commonly used antibody against the fluorescent tag protein. tGFP (turbo GFP) is an improved variant of GFP (green fluorescent protein) with bright green fluorescence, high pH-stability and photostability. The fast appearance of bright fluorescence of tGFP is critical for the applications of cell labeling, organelle labeling and promoter activity tracking. tGFP is used as an expression tag for OriGene's TrueORF cDNA clones (catalog numbers RG2xxxxx & MG2xxxxx).

  • Antibodies against Fluorescent Proteins

    OriGene offers the broad selection of antibodies against fluorescent proteins (FPs). These include mCherry, tdTomato, eGFP, turbo GFP, ZsGreen1, mKate, DsRed2, Timer, etc. Conjugations of these antibodies to HRP or magnetic beads are also available for Western blot, IP or protein purification. To view the full list of the FP antibodies, please click here.

Selected Citations of Anti-tag Antibodies

  • J. Wise, et al. Nucleolin inhibits Fas ligand binding and suppresses Fas-mediated apoptosis in vivo via a surface nucleolin-Fas complex. Blood, Jun 2013; 121: 4729 - 4739. [Anti-DDK]
  • H. Lei, et al. Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export. Nucleic Acids Res., Feb 2013; 41: 2517 - 2525. [Anti-eGFP]
  • K. Penicud, et al. DMAP1 is an essential regulator of ATM activity and function. Oncogene doi:10.1038. [Anti-tGFP]
  • F. Gao et al. A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function. Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [Anti-DDK]
  • Click here to view all citations

 

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