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Home Antibody Antitag Antibodies

Anti-tag Antibodies

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OriGene has developed a family of anti-tag antibodies to optimize the detection and purification of the recombinant proteins. Most of OriGene's anti-tag antibodies are mouse monoclonal antibodies which have been screened and extensively validated for high sensitivity and specificity.

Fusion Tag
DDK GST HA His Myc V5
Fluorescent Protein Tag
eGFP tGFP mCherry tdTomato More FP Tags
Tag Description Applications SKU Size Price  
ZsGreen1 Mouse monoclonal ZsGreen1 antibody, HRP conjugated WB TA183000 100ul $318 Add to Shopping Cart
ZsYellow1 Mouse monoclonal ZsYellow1 antibody, HRP conjugated WB TA183001 100ul $318 Add to Shopping Cart
DsRed2 Mouse monoclonal DsRed2 antibody, HRP conjugated WB TA183002 100ul $318 Add to Shopping Cart
Timer Mouse monoclonal Timer antibody, HRP conjugated WB TA183003 100ul $318 Add to Shopping Cart
tdTomato Mouse monoclonal tdTomato antibody, HRP conjugated WB 1:1000 TA183004 100ul $318 Add to Shopping Cart
AcGFP1 Mouse monoclonal AcGFP1/PS-CFP2 antibody, HRP conjugated WB TA183005 100ul $318 Add to Shopping Cart
DsRed-Express2 Mouse monoclonal DsRed-Express2 antibody, HRP conjugated WB TA183006 100ul $318 Add to Shopping Cart
mCherry Mouse monoclonal mCherry antibody, HRP conjugated WB TA183007 100ul $318 Add to Shopping Cart
mRaspberry Mouse monoclonal mRaspberry/AmCyan1 antibody, HRP conjugated WB TA183008 100ul $318 Add to Shopping Cart
mBanana Mouse monoclonal mBanana antibody, HRP conjugated WB TA183009 100ul $318 Add to Shopping Cart
mStrawberry Mouse monoclonal mStrawberry antibody, HRP conjugated WB TA183010 100ul $318 Add to Shopping Cart
eRFP Mouse monoclonal eRFP antibody, HRP conjugated WB TA183011 100ul $318 Add to Shopping Cart
mOrange Mouse monoclonal mOrange/mOrange2 antibody, HRP conjugated WB TA183012 100ul $318 Add to Shopping Cart
eCFP Mouse monoclonal eCFP antibody, HRP conjugated WB TA183013 100ul $318 Add to Shopping Cart
mPlum Mouse monoclonal mPlum antibody, HRP conjugated WB TA183014 100ul $318 Add to Shopping Cart
mGFP Mouse monoclonal mGFP/mCFP/mYFP/KillerRed antibody, HRP conjugated WB TA183015 100ul $318 Add to Shopping Cart
DsRed-Monomer Mouse monoclonal DsRed-Monomer antibody, HRP conjugated WB TA183016 100ul $318 Add to Shopping Cart
E2-Crimson Mouse monoclonal E2-Crimson antibody, HRP conjugated WB TA183017 100ul $318 Add to Shopping Cart
mKate Mouse monoclonal mKate antibody, HRP conjugated WB TA183018 100ul $318 Add to Shopping Cart
mBFP Mouse monoclonal mBFP antibody, HRP conjugated WB TA183019 100ul $318 Add to Shopping Cart
mRFP Mouse monoclonal mRFP antibody, HRP conjugated WB TA183020 100ul $318 Add to Shopping Cart
Dendra2 Mouse monoclonal Dendra2 antibody, HRP conjugated WB 1:1000 TA183021 100ul $318 Add to Shopping Cart
AsRed2 Mouse monoclonal AsRed2 antibody, HRP conjugated WB TA183022 100ul $318 Add to Shopping Cart
tGFP Mouse monoclonal tGFP antibody, HRP conjugated WB TA150043 100ul $318 Add to Shopping Cart
Myc 9E10, Anti-Myc tag monoclonal antibody, HRP WB TA150054 100ul $318 Add to Shopping Cart
eGFP Mouse monoclonal eGFP antibody, clone 5A2, HRP conjugated WB TA183051 100ul $318 Add to Shopping Cart
DDK 4C5, Anti-DDK Monoclonal antibody, HRP WB TA150030 100ul $318 Add to Shopping Cart
HA HA mouse monoclonal antibody, clone CB051, HRP conjugated WB TA183050 100ul $318 Add to Shopping Cart
* For bulk antibody package, please contact Tech Support
** Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C; Flag® is a trademark of Sigma-Aldrich.

Features of OriGene Antitag Antibodies

  • 4C5 Anti-DDK Antibody

    OriGene’s anti-tag antibodies are developed with high standards of production procedure and validation. For example, 4C5 anti-DDK antibody is generated to detect the DYKDDDDK epitope (same epitope as Flag®). This antibody has been verified for Western blot, immunoprecipitation, immunofluorescence and flow cytometry. Other features include:

    • Higher sensitivity and specificity compared to other vendors’ anti-flag antibodies
    • Can be used to purify recombinant proteins with high purities and yields
    • Can also be used to detect recombinant proteins with low expression levels

    4C5 anti-DDK antibody has already been applied to

    • Validate the recombinant protein expression from more than 15,000 OriGene TrueORF cDNA clones and VERIFY overexpression lysates
    • Purify more than 5,000 human recombinant proteins from HEK293 cells
    • Purify immunogens under native conditions for the development of TrueMAB antibodies
    AntiDDK DDK Comparison Image

    4C5 anti-DDK monoclonal antibody (TA50011) showed higher sensitivity in the side-by-side comparison with M2 monoclonal antibody from vendor S. Both antibodies were used at 1:2000 dilution against same sample (TP53) dilutions.

  • 2H8 Anti-tGFP Antibody

    2H8 anti-tGFP is another commonly used antibody against the fluorescent tag protein. tGFP (turbo GFP) is an improved variant of GFP (green fluorescent protein) with bright green fluorescence, high pH-stability and photostability. The fast appearance of bright fluorescence of tGFP is critical for the applications of cell labeling, organelle labeling and promoter activity tracking. tGFP is used as an expression tag for OriGene's TrueORF cDNA clones (catalog numbers RG2xxxxx & MG2xxxxx).

  • Antibodies against Fluorescent Proteins

    OriGene offers the broad selection of antibodies against fluorescent proteins (FPs). These include mCherry, tdTomato, eGFP, turbo GFP, ZsGreen1, mKate, DsRed2, Timer, etc. Conjugations of these antibodies to HRP or magnetic beads are also available for Western blot, IP or protein purification. To view the full list of the FP antibodies, please click here.

Selected Citations of Anti-tag Antibodies

  • J. Wise, et al. Nucleolin inhibits Fas ligand binding and suppresses Fas-mediated apoptosis in vivo via a surface nucleolin-Fas complex. Blood, Jun 2013; 121: 4729 - 4739. [Anti-DDK]
  • H. Lei, et al. Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export. Nucleic Acids Res., Feb 2013; 41: 2517 - 2525. [Anti-eGFP]
  • K. Penicud, et al. DMAP1 is an essential regulator of ATM activity and function. Oncogene doi:10.1038. [Anti-tGFP]
  • F. Gao et al. A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function. Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [Anti-DDK]
  • Click here to view all citations

 

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