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Home Antibody All anti-ATP6V1B1 antibodies

Anti-ATP6V1B1 TRUEMAB Antibody Clone OTI2D2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502667 ATP6V1B1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) 100ul $379 In Stock
LC400635 ATP6V1B1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502667 Carrier-free (BSA/glycerol-free) ATP6V1B1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(3)
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Also for ATP6V1B1 (NM_001692)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.
Clone NameClone OTI2D2 IsotypeIgG2b
Species ReactivityHuman Concentration0.47 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 56.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1)
Alternative NameATP6B1; RTA1B; VATB; VMA2; VPP3
Database LinkNP_001683
Entrez Gene 525 Human
FunctionThis gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].
Related PathwayDruggable Genome Oxidative phosphorylationMetabolic pathwaysVibrio cholerae infectionEpithelial cell signaling in Helicobacter pylori infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1B1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Anti-ATP6V1B1 mouse monoclonal antibody (TA502667) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1(RC209462 ).
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HEK293T cells transfected with either RC209462 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATP6V1B1 antibody(TA502667), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-ATP6V1B1 antibody(TA502667),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-ATP6V1B1 antibody(TA502667),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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