Anti-ATP5B Antibody, clone 5G2

Also for ATP5B (NM_001686)

OriGene Data

Immunogen	Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
Clone Name	clone 5G2	Isotype	IgG1
Species Reactivity	Human, Dog, Rat, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein
Alternative Name	ATPMB; ATPSB
Database Link	NP_001677
Function	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (TA500851, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (TA500851, Dilution 1:50)

IF Image

Anti-ATP5B mouse monoclonal antibody (TA500851) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B(RC201638).

IF Image

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (TA500851).

FC Image

HEK293T cells transfected with either pCMV6-ENTRY ATP5B(RC201638)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ATP5B mouse monoclonal(TA500851), and then analyzed by flow cytometry.

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OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-ATP5B TrueMAB Antibody clone 5G2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data