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Anti-ATP1A1 Antibody EP1845Y
Also for ATP1A1 (NM_000701)
|A synthetic peptide corresponding to residues near the N-terminus of human Na+/K+ ATPase ? was used as an immungen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:20000; FC: 1:20; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens ATPase, Na+/K+ transporting, alpha 1 polypeptide (ATP1A1), transcript variant 1|
|Na+, K+-ATPase is a heterodimeric enzyme responsible for the active maintenance of sodium and potassium gradients across the plasma membrane (1). The sodium- and potassium-dependent adenosine triphosphatase (Na+,K(+)-ATPase) maintains the transmembrane Na+ gradient to which is coupled all active cellular transport systems (2). It is well accepted that inhibition of the Na,K-ATPase in the heart, through effects on the Na/Ca exchanger, raises the intracellular Ca2+ concentration and strengthens cardiac contraction. Assessing the phenotypes of mouse hearts with genetically reduced levels of Na,K-ATPase alpha 1 or alpha 2 isoforms clearly demonstrates different functional roles for these isoforms in vivo. Results definitively illustrate a specific role for the alpha 2 Na,K-ATPase isoform in Ca2+ signaling during cardiac contraction (3). |
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Western blot - Sodium Potassium ATPase antibody [EP1845Y]; Anti-Sodium Potassium ATPase [EP1845Y] antibody at 1/20000 dilution + Hela cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 113 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sodium Potassium ATPase antibody [EP1845Y]; ab76020, at a 1/100 dilution, staining Sodium Potassium ATPase in paraffin embedded human stomach carcinoma tissue by Immunohistochemistry. An HRP/AP polymerized secondary antibody was also used.
Flow Cytometry - Anti-Sodium Potassium ATPase antibody [EP1845Y]; Overlay histogram showing HeLa cells stained with ab76020 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.