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Anti-ATG9A Antibody EPR2450(2)
Also for ATG9A (NM_024085)
|A synthetic peptide corresponding to residues in human ATG9A was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens autophagy related 9A (ATG9A), transcript variant 2|
|APG9L1; mATG9; MGD3208|
Entrez Gene 79065 Human
Entrez Gene 245860 Mouse
Entrez Gene 363254 Rat
|Autophagy is a degradation system of cytoplasmic proteins and organelles via formation of double-membrane vesicles called autophagosomes (1). Defects in autophagy are associated with various human diseases including cancer and neurodegeneration (2). ATG9 functions in autophagy as the only integral membrane protein that is essential for double-membrane vesicle formation (2). It is probably transported to the site of autophagosome formation, the pre-autophagosomal structure (PAS) (2). One member of the ATG9 family, ATG9A (Autophagy-related gene 9A), is an autophagy-related membrane protein that is required for autophagosome formation (3, 4). Although ATG9A is ubiquitously detected, it is highly expressed in neurons of the central nervous system (4).|
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Western blot - ATG9A antibody [EPR2450(2)]; All lanes : Anti-ATG9A antibody [EPR2450(2)] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A375 cell lysate.Lane 4 : Mouse brain cell lysate.Lane 5 : Rat brain cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 94 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATG9A antibody [EPR2450(2)]; TA307050, at 1/100, staining ATG9A in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - ATG9A antibody [EPR2450(2)]; TA307050, at 1/50, staining ATG9A in HepG2 cells by Immunofluorescence.