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Anti-ATG3 TRUEMAB Antibody clone 3H2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503346
  • ATG3 mouse monoclonal antibody,clone 3H2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC411559) , 20ug
100ul 379 In Stock
CF503346
  • Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody,clone 3H2
100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(1)
FC(2)
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Also for ATG3 (NM_022488)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
Clone Nameclone 3H2 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.79 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens autophagy related 3 (ATG3), transcript variant 1
Alternative NameAPG3; APG3-LIKE; APG3L; PC3-96
Database LinkNP_071933
FunctionAutophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM, Mar 2008].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503346)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503346)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503346)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503346)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503346)
IF Image
Anti-ATG3 mouse monoclonal antibody (TA503346) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453).
FC Image
Flow cytometric Analysis of Hela cells, using anti-ATG3 antibody(TA503346),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-ATG3 antibody(TA503346),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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