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Anti-ATG3 TRUEMAB Antibody clone 3C6
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for ATG3 (NM_022488)
|Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.|
|Human , Dog , Rat , Monkey
|WB, IF, FC
|| WB 1:500~2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens autophagy related 3 (ATG3), transcript variant 1|
|APG3; APG3-LIKE; APG3L; PC3-96|
Entrez Gene 64422 Human
Entrez Gene 171415 Rat
Entrez Gene 708305 Monkey
Entrez Gene 478564 Dog
|Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM, Mar 2008]. |
| Regulation of autophagy|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-ATG3 mouse monoclonal antibody (TA503301) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453).
HEK293T cells transfected with either RC203453 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATG3 antibody(TA503301), and then analyzed by flow cytometry.