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Anti-ATF1 PHOSPHO Antibody EP1591(2)Y
Also for ATF1 (NM_005171)
|A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF-1 was used as an immunogen. This antibody detects ATF-1 phosphorylated on serine 63|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||ELISA: Use at an assay dependent concentration; WB: 1:50000 - 1:100000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens activating transcription factor 1 (ATF1)|
|EWS-ATF1; FUS/ATF-1; TREB36|
|ATF-1 is a cAMP-responsive enhancer (CRE) binding protein that is expressed in a wide variety of cell types and is capable of dimerizing with CREB. ATF-1 contains a consensus phosphorylation site for PKA and shares extensive homology with CREB in the region surrounding and carboxyl-terminal to the PKA site (1). Epidermal growth factor induction of c-jun expression requires ATF-1 and MEF2 sites in the c-jun promoter. Activation of the c-jun promoter through the ATF-1 site requires phosphorylation of ATF-1 at serine 63. Two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and the ERK pathway acts through downstream phosphorylation of ATF-1 (2). |
EGFR1 Signaling Pathway
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Western blot - ATF1 (phospho S63) antibody [EP1591(2)Y]; All lanes : Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] at 1/100000 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.goat anit rabbit HRP labelled IgG at 1/2000 dilution.Predicted band size : 29 kDa.Observed band size : 29 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATF1 (phospho S63) antibody [EP1591(2)Y]; Immunohistochemical analysis of ATF1 in paraffin embedded human breast carcinoma tissue, using TA301291 at a 1/100 dilution.