Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas Blood, Mar 2013; 121: 2563 - 2566.
[anti-HA]
Regulation of the PI3-K/Akt Survival Pathway in the Rat Endometrium Biol Reprod, Mar 2013; 88: 79.
[Akt3]
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt159.
[LA]
Ruxolitinib as potential targeted therapy for patients with JAK2 rearrangements Haematologica, Mar 2013; 98: 404 - 408.
[JAK2]
Homo sapiens activating transcription factor 1 (ATF1)
Synonyms:
EWS-ATF1; FUS/ATF-1; TREB36
Immunogen
A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF-1 was used as an immunogen. This antibody detects ATF-1 phosphorylated on serine 63
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
ATF-1 is a cAMP-responsive enhancer (CRE) binding protein that is expressed in a wide variety of cell types and is capable of dimerizing with CREB. ATF-1 contains a consensus phosphorylation site for PKA and shares extensive homology with CREB in the region surrounding and carboxyl-terminal to the PKA site (1). Epidermal growth factor induction of c-jun expression requires ATF-1 and MEF2 sites in the c-jun promoter. Activation of the c-jun promoter through the ATF-1 site requires phosphorylation of ATF-1 at serine 63. Two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and the ERK pathway acts through downstream phosphorylation of ATF-1 (2).
Related Pathway
EGFR1 Signaling Pathway
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Western blot analysis on HeLa cell lysates using anti-Phospho-ATF-1 (pS63), 1:100,000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.
