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Home Antibody All anti-ASL antibodies

Anti-ASL Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308495
  • Rabbit polyclonal antibody to Argininosuccinate Lyase (argininosuccinate lyase)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424953) , 20ug Explanation
100ul 325 3-7 Days
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WB(2)
IHC(2)
IF(1)
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Also for ASL (NM_000048)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 13 and 261 of ASL (Uniprot ID#P04424)
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 52kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.

Reference Data

Target NameHomo sapiens argininosuccinate lyase (ASL), transcript variant 2
Alternative NameASAL
Database LinkNP_000039
Entrez Gene 435 Human
FunctionThis gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
Related Pathway Alanine, aspartate and glutamate metabolismArginine and proline metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
ASL antibody detects ASL protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lys
WB Image
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 10% SDS PAGE. TA308495 diluted at 1:1000.
IHC Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASL(TA308495) antibody at 1:500 dilution.
IHC Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ASL(TA308495) antibody at 1:500 dilution.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Argininosuccinate Lyase(TA308495) antibody at 1:200 dilution.

 

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