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Anti-ARRB1 Antibody E274
Also for ARRB1 (NM_020251)
|A synthetic peptide corresponding to residues near N-terminus of human ß-arrestin-1 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use at an assay dependent concentration; WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC: 1:100; FC: 1:10 - 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens arrestin, beta 1 (ARRB1), transcript variant 2|
|Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described, however, their exact functions are not known. [provided by RefSeq]. |
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - beta Arrestin 1 antibody [E274]; Anti-beta Arrestin 1 antibody [E274] at 1/500 dilution + 293 cell lysate.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Paraffin-embedded sections) - beta Arrestin 1 antibody [E274]; ab32099 at a 1:100 dilution staining beta Arrestin 1 in human lymph node using Immunohistochemistry, Paraffin Embedded Tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Arrestin 1 antibody [E274]; ab32099 stainingbeta Arrestin 1in humanprostate carcinomatissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Tris/EDTA pH9.0 and then blockingwith 1% donkey serumfor1 hourat 20°C was performed. The primary antibody was diluted 1/100and incubated with sample for1 hour at 20°C in PBS. ABiotin conjugateddonkey polyclonal to rabbit IgG was used undilutedas secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-beta Arrestin 1 antibody [E274]; ab32099 staining beta Arrestin 1 in C4-2B (Human prostate cancer cell line) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in diluent) for 1 hour at 21°C. An AlexaFluor?488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
Flow Cytometry-beta Arrestin 1 antibody [E274](ab32099); Overlay histogram showing PC3 cells stained with ab32099 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.