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Anti-ARPC5 Antibody EP1551Y
Also for ARPC5 (NM_005717)
|A synthetic peptide corresponding to residues on the N-terminus of human p16-ARC was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||ICC/IF: 1:50 - 1:100; FC: 1:50; IP: 1:70; WB: 1:20000; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5), transcript variant 1|
|ARC16; dJ127C7.3; p16-Arc|
|This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end. |
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Western blot - p16 ARC antibody [EP1551Y]; Anti-p16 ARC antibody [EP1551Y] at 1/20000 dilution + Fetal brain lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 16 kDa.
Immunohistochemistry (Paraffin-embedded sections) - p16 ARC antibody [EP1551Y]; Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.
Flow Cytometry - p16 ARC antibody [EP1551Y]; Overlay histogram showing HeLa cells stained with ab51243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.