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Home Antibody All anti-ARPC5 antibodies

Anti-ARPC5 Antibody EP1551Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303704
  • Rabbit monoclonal antibody against p16-Arc (N-term) (EP1551Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417115) , 20ug Explanation
100uL 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for ARPC5 (NM_005717)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human p16-ARC was used as an immunogen.
Clone NameEP1551Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsICC/IF: 1:50 - 1:100; FC: 1:50; IP: 1:70; WB: 1:20000; IHC-P: Use at an assay dependent dilution
Predicted MW Explanation 16 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens actin related protein 2/3 complex, subunit 5, 16kDa (ARPC5), transcript variant 1
Alternative NameARC16; dJ127C7.3; p16-Arc
Database LinkNP_005708
Entrez Gene 10092 Human
Entrez Gene 67771 Mouse
Entrez Gene 360854 Rat
FunctionThis gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Related Pathway Fc gamma R-mediated phagocytosisRegulation of actin cytoskeletonPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - p16 ARC antibody [EP1551Y]; Anti-p16 ARC antibody [EP1551Y] at 1/20000 dilution + Fetal brain lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 16 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - p16 ARC antibody [EP1551Y]; Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.
FC Image
Flow Cytometry - p16 ARC antibody [EP1551Y]; Overlay histogram showing HeLa cells stained with TA303704 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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