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Anti-ARHGAP5 Antibody EP489Y
Also for ARHGAP5 (NM_001173)
|A synthetic peptide corresponding to residues in human p190-B RhoGAP was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IHC-P: Use at an assay dependent concentration; FC: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Is unsuitable for ICC or IP.
|Homo sapiens Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2|
|GFI2; p190-B; p190BRhoGAP; RhoGAP5|
|The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3). |
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Western blot - RhoGAP antibody [EP489Y]; All lanes : Anti-RhoGAP antibody [EP489Y] at 1/500 dilution.Lane 1 : Hela cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : MCF-7 cell lysate.Lane 4 : K562 cell lysate.Lane 5 : A431 cell lysate.Lane 6 : 293 cell lysate.Lane 7 : NIH 3T3 cell lysate.Lane 8 : PC12 cell lysate.Predicted band size : 171 kDa.Observed band size : 190 kDa .
Immunohistochemistry (Paraffin-embedded sections) - RhoGAP antibody [EP489Y]; ab32328, at a 1/100 dilution, staining human RhoGAP in stomach carcinoma by Immunohistochemistry, Paraffin embedded tissue