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Anti-ARHGAP25 TRUEMAB Antibody Clone OTI12E6
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for ARHGAP25 (NM_014882)
|Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2|
Entrez Gene 9938 Human
|ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (RC217414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501744, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501744, Dilution 1:50)
Anti-ARHGAP25 mouse monoclonal antibody (TA501744) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25(RC217414).
HEK293T cells transfected with either RC217414 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGAP25 antibody(TA501744), and then analyzed by flow cytometry.