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Home Antibody All anti-ARHGAP22 antibodies
UltraMAB

Anti-ARHGAP22 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590468
  • Rabbit Polyclonal ARHGAP22 Antibody
100ug $325 In Stock
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WB(2)
IHC(11)
IF(1)
ELISA(1)
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Also for ARHGAP22 (NM_021226)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration0.8mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens Rho GTPase activating protein 22 (ARHGAP22), transcript variant 3
Alternative NameRhoGAP2; RhoGap22
Database LinkNP_067049
FunctionARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP22 (RC220614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP22.
WB Image
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA Image
ELISA: ARHGAP22 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ARHGAP22 Rabbit Polyclonal antibody. (TA590468)
IHC Image
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
IF Image
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & plasma membrane.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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