Anti-ARFGAP1 Antibody, clone 2C5

Also for ARFGAP1 (NM_175609)

OriGene Data

Immunogen	Full length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
Clone Name	clone 2C5	Isotype	IgG2b
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2
Alternative Name	ARF1GAP; HRIHFB2281
Database Link	NP_783202
Function	The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.

IF Image

Anti-ARFGAP1 mouse monoclonal antibody (TA502626) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1(RC206987).

FC Image

HEK293T cells transfected with either RC206987 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARFGAP1 antibody(TA502626), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-ARFGAP1 antibody(TA502626),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-ARFGAP1 antibody(TA502626),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-ARFGAP1 TrueMAB Antibody clone 2C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data